CAPILLARY TUBE METHOD FOR COUNTING VIABLE BACTERIA

Filter Method for Counting Viable Organisms

The membrane filter, also known as molecular filter, is valuable for its ability to permit passage of large volumes of fluid and yet retain on its surface particles of bacterial size. The history of its development and some of its properties have been reviewed by Goetz and Tsuneishi (1951). Other properties and specifications are available from the manufacturers.3 At present details of manufact...

A method for counting acid-fast bacteria.

A reliable, convenient, and accurate method for counting ac id-fast hacteria ( AFB) is essential for much work with Mycobacterium leprae. The procedure used , previously in the laboratory (~, 4 ) had two technical difficulties: (a) In order for the drops to spread satisfactorily the microscope slides had to have a uniform wetability of the correct degree; sometimes this requirement was difficul...

Simplified agar plate method for quantifying viable bacteria.

We have developed a simple and reproducible technique that we have termed “track dilution” for enumerating viable bacteria, which reduces time and materials required compared to standard agar plate colony counting methods. Many microbiological applications require an accurate determination of bacterial numbers prior to inoculation into, or subsequent to their isolation from, experimental models...

New method for counting bacteria associated with coral mucus.

The ability to count bacteria associated with reef-building corals in a rapid, reliable, and cost-effective manner has been hindered by the viscous and highly autofluorescent nature of the coral mucus layer (CML) in which they live. We present a new method that disperses bacterial cells by trypsinization prior to 4',6-diamidino-2-phenylindole (DAPI) staining and quantification by epifluorescenc...

A Quantitative Differential Method for Counting Mixed Cultures of Bacteria.